New information: FR475 ready to use PCR kit to detect Wuhan / wild strain and mutations of SARS CoV-2 in one step+++ FR475 ready to use PCR kit to detect SARS CoV-2 and its mutated versions in one step is only solution on the world, which can help to stop the pandemic outbreak very quickly as this kit is simple to use and it does not give hardly any false results, therefore one can isolate the positive cases, while the negative cases can work and move freely. The different mutations e.g., BA.4/5 and BA.2.7 along with other mutations do not affect the performance of this kit against the approved kits, which are made on the basis of N and E genes, where the mutations are also taking place and making impossible to give the correct results, moreover the multiplex kits for 3 targets are prone to give wrong results as this is well known fact in literature and in the laboratories. Therefore, approving such kits through FDA, WHO and CE are not contributing the stop the pandemic outbreak in wished way. In April, 2020 many countries around the world used FR 475, one can see that these countries had full check on the outbreak. The outbreak starts increasing as the poor-quality approved kits were brought on the market in 2020 through many including big biotech companies. Not only this, questionable vaccines are approved around the world, now many countries are preparing for 4th booster dose, which will be also little use for all of us except some persons as winter is approaching and the cases are going to increase. Very high number of vaccinated get reinfection, if they do not take precautions like mask wearing and distancing, which should likely to provide long term immunity, but chances are very high that some reinfected cases are likely to get complications of reinfections. If you really want to control the pandemic outbreak, one should use FR 475. Another advantage of this kit is that it is same version of the kit in Feb, 2020 and today. It means one can conduct research on the old samples, which were tested with other kits in the past. This research work may be given as master or doctorate work around the world. Many approved kits have been removed from the approval list of FDA and WHO as this shows that many companies are not able to make any good kit because of lack of deep knowledge of virology. Such companies developed their kits on basis of information of primers and probes provided WHO in 2020, which were not of good quality to develop good and reliable kits. It is impossible to do the gene sequencing and next generation sequencing of each sample. If any Govt wants to reduce the pandemic outbreak, the answer is FR475. They should take a few of this kit and compare the results and after that Govt can approve this kit for clinical use. If the world wants to waste another year and huge money, please carry on with so called approved kits. There is list of mutations.
BA.2.7 NSP1 S135R NSP3 T24l G489S NSP4 L264F T327l L438F T492l NSP5 P132H NSP6 106-107del NSP12 P323L NSP13 R392C NSP14 142V NSP15 T112l S T19l L24S 25-27del G142D V213G S371F S373P S375F T376A D405N R408S K417N N440K S477N T478K E484A Q498R N501Y Y505H D614G H655Y N679K P681HN764K D796Y O954H N969K Orf3a T223l E T91 M A63T Orf6 D61L Orf8 S84L N P13L 31-33del R203K G204R NSP3 S403L P822S NSP6 F108L NSP8 N 118S NSP12 G671S S K147E W152R F157L l210V G257S G339H G446S N460K E T11A M Q19E
BA.4/5 NSP1 S135R NSP3 T24l G489S NSP4 L264F T327l L438F T492l NSP5 P132H NSP6 106-107del NSP12 P323L NSP13 R392C NSP14 142V NSP15 T112l S T19l L24S 25-27del G142D V213G S371F S373P S375F T376A D405N R408S K417N N440K S477N T478K E484A Q498R N501Y Y505H D614G H655Y N679K P681HN764K D796Y O954H N969K Orf3a T223l E T91 M A63T Orf6 D61L Orf8 S84L N P13L 31-33del R203K G204R NSP1 141-143del NSP6 108del S 69-70del G339D L452R G486V Q493R M D3N N P151S